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    Ribosome Biogenesis Methods and Protocols /

    Published 2022
    Table of Contents: “…A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity across the Tree of Life -- Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes -- Analysis of Yeast RNAP I Transcription of Nucleosomal Templates In Vitro -- Specialization of RNA Polymerase I in Comparison to Other Nuclear RNA Polymerases of Saccharomyces cerevisiae -- Structural Studies of Eukaryotic RNA Polymerase I using Cryo-Electron Microscopy -- Preparation of RNA Polymerase Complexes for their Analysis by Single Particle Cryo-Electron Microscopy -- Eukaryotic Ribosome Assembly and Nucleo-Cytoplasmic Transport -- Tethered MNase Structure Probing as Versatile Technique for Analyzing RNPs using Tagging Cassettes for Homologous Recombination in Saccharomyces cerevisiae -- Chemical Modifications of Ribosomal RNA -- In Vitro Selection of Deoxyribozymes for the Detection of RNA Modifications -- Mapping of the Chemical Modificationsof rRNAs -- Non-Radioactive In Vivo Labelling of RNA with 4-thio-uracil -- Translation Phases in Eukaryotes -- Differential Translation Activity using Bio-Orthogonal Non-Canonical Amino Acids Tagging (BONCAT) in Archaea -- Thermofluor-Based Analysis of Protein Integrity and Ligand Interactions -- In Vitro Assembly of a Fully Reconstituted Yeast Translation System for Studies of Initiation and Elongation Phases of Protein Synthesis.…”
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    Electronic eBook
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