Search Results - 種痘~

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    Cyborgs in Latin America by Brown, J.

    Published 2010
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    Electronic eBook
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    History and Cultural Memory in Neo-Victorian Fiction Victorian Afterimages / by Mitchell, Kate

    Published 2010
    Subjects: “…Literature, Modern-20th century.…”
    Link to Metadata
    Electronic eBook
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    Accountability in Public Policy Partnerships by Steets, J.

    Published 2010
    Link to Metadata
    Electronic eBook
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    Networks in the Russian Market Economy by Lonkila, M.

    Published 2011
    Link to Metadata
    Electronic eBook
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    Determinants of Financial Development by Huang, Y.

    Published 2011
    Link to Metadata
    Electronic eBook
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    De La Mettrie's Ghost The Story Of Decisions / by Nunn, C.

    Published 2005
    Link to Metadata
    Electronic eBook
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    Statistical Population Genomics

    Published 2020
    Table of Contents: “…A Population Genomics Lexicon -- Processing and Analyzing Multiple Genomes Alignments with MafFilter -- Data Management and Summary Statistics with PLINK -- Exploring Population Structure with Admixture Models and Principal Components Analysis -- Detecting Positive Selection in Populations using Genetic Data -- polyDFE: Inferring the Distribution of Fitness Effects and Properties of Beneficial Mutations from Polymorphism Data -- MSMC and MSMC2: The Multiple Sequentially Markovian Coalescent.…”
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    Electronic eBook
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    Preclinical MRI of the Kidney Methods and Protocols /

    Published 2021
    Table of Contents: “…Recommendations for Preclinical Renal MRI: A Comprehensive Open-Access Protocol Collection to Improve Training, Reproducibility, and Comparability of Studies -- Animal Models of Renal Pathophysiology and Disease -- Preparation and Monitoring of Small Animals in Renal MRI -- Reversible (Patho-)Physiologically Relevant Test Interventions: Rationale and Examples -- Preparation of Ex Vivo Rodent Phantoms for Developing, Testing, and Training MR Imaging of the Kidney and Other Organs -- Quantitative Assessment of Renal Perfusion and Oxygenation by Invasive Probes: Basic Concepts -- Ultrasound and Photoacoustic Imaging of the Kidney: Basic Concepts and Protocols -- Hardware Considerations for Preclinical Magnetic Resonance of the Kidney -- MRI Mapping of Renal T1: Basic Concept -- MRI Mapping of the Blood Oxygenation Sensitive Parameter T2* in the Kidney: Basic Concept -- Renal Diffusion Weighted Imaging (DWI) for Apparent Diffusion Coefficient (ADC), Intra Voxel Incoherent Motion (IVIM), and Diffusion Tensor Imaging (DTI): Basic Concept -- Dynamic Contrast Enhancement (DCE)-MRI Derived Renal Perfusion and Filtration: Basic Concepts -- Non-Invasive Renal Perfusion Measurement Using Arterial Spin Labelling (ASL) MRI: Basic Concept -- Renal pH Imaging Using Chemical Exchange Saturation Transfer (CEST)-MRI: Basic Concepts -- Sodium (23Na) MRI of the Kidney: Basic Concept -- Hyperpolarized Carbon (13C) MRI of the Kidneys: Basic Concepts -- Functional Imaging Using Fluorine (19F) MR Methods: Basic Concepts -- MR Elastography of the Abdomen: Basic Concepts -- Monitoring Renal Hemodynamics and Oxygenation by Invasive Probes: Experimental Protocol -- Essential Practical Steps for MRI of the Kidney in Experimental Research -- Assessment of Renal Volume with MRI: Experimental Protocol -- Experimental Protocols for MRI Mapping of Renal T1 -- Experimental Protocols for MRI Mapping of the Blood Oxygenation Sensitive Parameters T2* and T2 in the Kidney -- Renal MRI Diffusion: Experimental Protocol -- Dynamic Contrast Enhanced (DCE)-MRI Derived Renal Perfusion and Filtration: Experimental Protocol -- Renal Blood Flow Using Arterial Spin Labeling (ASL)-MRI: Experimental Protocol and Principles -- Renal pH Mapping Using Chemical Exchange Saturation Transfer (CEST)-MRI: Experimental Protocol -- Sodium (23Na) MRI of the Kidney: Experimental Protocol -- Hyperpolarized Carbon (13C) MRI of the Kidney: Experimental Protocol -- Fluorine (19F) MRI for Assessing Inflammatory Cells in the Kidney: Experimental Protocol -- Fluorine (19F) MRI to Measure Renal Oxygen Tension and Blood Volume: Experimental Protocol -- MR Elastography of the Abdomen: Experimental Protocols -- Subsegmentation of the Kidney in Experimental MR Images Using Morphology-Based Regions-of-Interest or Multiple-Layer Concentric Objects -- De-Noising for Improved Parametric MRIof the Kidney: Protocol for Non-Local Means Filtering -- Analysis Protocols for MRI Mapping of Renal T1 -- Analysis Protocols for MRI Mapping of the Blood Oxygenation Sensitive Parameters T2* and T2 in the Kidney -- Analysis of Renal Diffusion Weighted Imaging (DWI) Using Apparent Diffusion Coefficient (ADC) and Intra Voxel Incoherent Motion (IVIM) Models -- Analysis Protocol for Dynamic Contrast Enhanced (DCE)-MRI of Renal Perfusion and Filtration -- Quantitative Analysis of Renal Perfusion by Arterial Spin Labeling -- Analysis Protocol for the Quantification of Renal pH Using Chemical Exchange Saturation Transfer (CEST)-MRI -- Analysis Protocol for Renal Sodium (23Na) MR Imaging -- Analysis Methods for Hyperpolarized Carbon (13C) MRI of the Kidney -- Data Preparation Protocol for Low Signal-to-Noise Ratio Fluorine-19 MRI.…”
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    Electronic eBook
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    Antisense RNA Design, Delivery, and Analysis

    Published 2022
    Table of Contents: “…Introduction and History of the Chemistry of Nucleic Acids Therapeutics -- Antisense RNA Therapeutics: A Brief Overview -- Design of Bifunctional Antisense Oligonucleotides for Exon Inclusion -- Design and Delivery of SINEUP: A New Modular Tool to Increase Protein Translation -- How to Design U1 snRNA Molecules for Splicing Rescue -- Conjugation of Nucleic Acids and Drugs to Gold Nanoparticles -- Determination of Optimum Ratio of Cationic Polymers and Small Interfering RNA with Agarose Gel Retardation Assay -- Generation of Protein-Phosphorodiamidate Morpholino Oligomer Conjugates for Efficient Cellular Delivery via Anthrax Protective Antigen -- Development and Use of Cellular Systems to Assess and Correct Splicing Defects -- Modeling Splicing Variants Amenable to Antisense Therapy by Use of CRISPR-Cas9-Based Gene Editing in HepG2 Cells -- In Vitro Models for the Evaluation of Antisense Oligonucleotides in Skin -- In Vitro Delivery of PMOs in Myoblasts by Electroporation -- Rapid Determination of MBNL1 Protein Levels by Quantitative Dot Blot for Evaluation of Antisense Oligonucleotides in Myotonic Dystrophy Myoblasts -- Evaluation of Exon Skipping and Dystrophin Restoration in In Vitro Models of Duchenne Muscular Dystrophy -- Generation of Human iPSC-Derived Myotubes to Investigate RNA-Based Therapies In Vitro -- Eye on a Dish Models to Evaluate Splicing Modulation -- Establishment of In Vitro Brain Models for AON Delivery -- Considerations for Generating Humanized Mouse Models to Test Efficacy of Antisense Oligonucleotides -- Generation of Humanized Zebrafish Models for the In Vivo Assessment of Antisense Oligonucleotide-Based Splice Modulation Therapies -- Use of Small Animal Models for Duchenne and Parameters to Assess Efficiency upon Antisense Treatment -- In Vivo Models for the Evaluation of Antisense Oligonucleotides in Skin -- Delivery of Antisense Oligonucleotides to the Mouse Retina -- Delivery of Antisense Oligonucleotides to the Mouse Brain by Intracerebroventricular Injections -- Intrathecal Delivery of Therapeutic Oligonucleotides for Potent Modulation of Gene Expression in the Central Nervous System -- Preclinical Safety Assessment of Therapeutic Oligonucleotides -- Preclinical Evaluation of the Renal Toxicity of Oligonucleotide Therapeutics in Mice -- Protocol for Isolation and Culture of Mouse Hepatocytes (HCs), Kupffer Cells (KCs), and Liver Sinusoidal Endothelial Cells (LSECs) in Analyses of Hepatic Drug Distribution -- Patent Considerations When Embarking on New Antisense Drug Programs.…”
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    Electronic eBook
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    Immunogenetics Methods and Protocols /

    Published 2022
    Table of Contents: “…The Advent of Precision Immunology: Immunogenetics at the Center of Immune Cell Analysis in Health and Disease -- Next-Generation Sequencing-Based Clonality Detection of Immunoglobulin Gene Rearrangements in B-Cell Lymphoma -- One-Step Next Generation Sequencing of Immunoglobulin and T-Cell Receptor Gene Recombinations for MRD Marker Identification in Acute Lymphoblastic Leukemia -- Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq -- Minimal Residual Disease Analysis by Monitoring Immunoglobulin and T-Cell Receptor Gene Rearrangements by Quantitative PCR and Droplet Digital PCR -- Quality Control for IG/TR Marker Identification and MRD Analysis -- cfDNA-Based NGS IG Analysis in Lymphoma -- Targeted Locus Amplification as Marker Screening Approach to Detect Immunoglobulin (IG) Translocations in B-Cell Non-Hodgkin Lymphomas -- Immunoglobulin/T-Cell Receptor Capture Strategy for Comprehensive Immunogenetics -- Immunoglobulin Gene Mutational Status Assessment by Next Generation Sequencing in Chronic Lymphocytic Leukemia -- NGS-Based B-Cell Receptor Repertoire Analysis in the Context of Inborn Errors of Immunity -- Generic Multiplex Digital PCR for Accurate Quantification of T-Cells in Copy Number Stable and Unstable DNA Samples -- Gene Engineering T-Cells with T-Cell Receptor for Adoptive Therapy -- Combined Analysis of Transcriptome and T-Cell Receptor Alpha Beta (TRA/TRB) Repertoire in Paucicellular Samples at the Single Cell Level -- AIRR Community Guide to Planning and Performing AIRR-Seq Experiments -- Adaptive Immune Receptor Repertoires (AIRR) Community Guide to TR and IG Gene Annotation -- Adaptive Immune Receptor Repertoires (AIRR) Community Guide to Repertoire Analysis -- Bulk gDNA Sequencing of Antibody Heavy Chain Gene Rearrangements for Detection and Analysis of B-Cell Clone Distribution: A Method by the AIRR Community -- Bulk Sequencing from mRNA with UMI for Evaluation of B-Cell Isotype and Clonal Evolution: A Method by the AIRR Community -- Single Cell Analysis and Tracking of Antigen-Specific T-Cells: Integrating Paired-Chain AIRR-Seq and Transcriptome Sequencing: A Method by the AIRR Community -- Quality Control: Chain Pairing Precision and Monitoring of Cross-Sample Contamination: A Method by the AIRR Community -- Immune Repertoire Analysis on High-Performance Computing Using VDJServer V1: A Method by the AIRR Community -- Data Sharing and Re-Use: A Method by the AIRR Community -- IMGT® Immunoinformatics Tools for Standardized V-DOMAIN Analysis -- IMGT/3Dstructure-DB: T-Cell Receptor TR Paratope and Peptide/Major Histocompatibility pMH Contact Sites and Epitope -- ARResT/Interrogate Immunoprofiling Platform: Concepts, Workflows, and Insights -- Purpose-Built Immunoinformatics for BcR IG/TR Repertoire Data Analysis.…”
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    Electronic eBook
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    Whole-Body Regeneration Methods and Protocols /

    Published 2022
    Table of Contents: “…-Studying Tunicata WBR using Botrylloides anceps -- In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis -- Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyi -- Analysis of Spatial Gene Expression at the Cellular Level in Stony Corals -- Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow Cytometry -- Non-Invasive Intra-Vascular Micro-Transfusion in Colonial Tunicates -- Gene Manipulation in Hydractinia -- Manipulation of Gene Activity in the Regenerative Model Sea Anemone, Nematostella vectensis -- Monitoring Telomere Maintenance during Regeneration of Annelids -- Analysis of DNA Double Stranded Breaks using the COMET Assay in Planarians -- Random Integration Transgenesis in a Free-Living Regenerative Flatworm Macrostomum lignano -- RNAi Screening to Assess Tissue Regeneration in Planarians -- Monitoring Chromatin Regulation in Planarians using Chromatin Immunoprecipitation followed by Sequencing (ChIP-seq) -- Assessing Chromatin Accessibility during WBR in Acoels -- Single Cell Transcriptomic Analysis in the Regenerating Cnidarian Nematostella vectensis -- Characterization of Soluble Cell-Free Coelomic Fluid Proteome from the Starfish Marthasterias glacialis -- Using RNA-Sequencing for Transcriptome Profiling of Botrylloides sp. …”
    Link to Metadata
    Electronic eBook
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    Ribosome Biogenesis Methods and Protocols /

    Published 2022
    Table of Contents: “…A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity across the Tree of Life -- Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes -- Analysis of Yeast RNAP I Transcription of Nucleosomal Templates In Vitro -- Specialization of RNA Polymerase I in Comparison to Other Nuclear RNA Polymerases of Saccharomyces cerevisiae -- Structural Studies of Eukaryotic RNA Polymerase I using Cryo-Electron Microscopy -- Preparation of RNA Polymerase Complexes for their Analysis by Single Particle Cryo-Electron Microscopy -- Eukaryotic Ribosome Assembly and Nucleo-Cytoplasmic Transport -- Tethered MNase Structure Probing as Versatile Technique for Analyzing RNPs using Tagging Cassettes for Homologous Recombination in Saccharomyces cerevisiae -- Chemical Modifications of Ribosomal RNA -- In Vitro Selection of Deoxyribozymes for the Detection of RNA Modifications -- Mapping of the Chemical Modificationsof rRNAs -- Non-Radioactive In Vivo Labelling of RNA with 4-thio-uracil -- Translation Phases in Eukaryotes -- Differential Translation Activity using Bio-Orthogonal Non-Canonical Amino Acids Tagging (BONCAT) in Archaea -- Thermofluor-Based Analysis of Protein Integrity and Ligand Interactions -- In Vitro Assembly of a Fully Reconstituted Yeast Translation System for Studies of Initiation and Elongation Phases of Protein Synthesis.…”
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    Electronic eBook