Ribosome Biogenesis Methods and Protocols /
This Open Access volume provides comprehensive reviews and describes the latest techniques to study eukaryotic ribosome biogenesis. For more than 50 years ribosomes are a major research topic. Our knowledge about ribosome biogenesis and function such as transcription, mRNA modification, and translat...
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| Format: | Electronic eBook |
| Language: | English |
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New York, NY :
Springer US : Imprint: Humana,
2022.
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| Edition: | 1st ed. 2022. |
| Series: | Methods in Molecular Biology,
2533 |
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| Online Access: | Link to Metadata |
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Table of Contents:
- A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity across the Tree of Life
- Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes
- Analysis of Yeast RNAP I Transcription of Nucleosomal Templates In Vitro
- Specialization of RNA Polymerase I in Comparison to Other Nuclear RNA Polymerases of Saccharomyces cerevisiae
- Structural Studies of Eukaryotic RNA Polymerase I using Cryo-Electron Microscopy
- Preparation of RNA Polymerase Complexes for their Analysis by Single Particle Cryo-Electron Microscopy
- Eukaryotic Ribosome Assembly and Nucleo-Cytoplasmic Transport
- Tethered MNase Structure Probing as Versatile Technique for Analyzing RNPs using Tagging Cassettes for Homologous Recombination in Saccharomyces cerevisiae
- Chemical Modifications of Ribosomal RNA
- In Vitro Selection of Deoxyribozymes for the Detection of RNA Modifications
- Mapping of the Chemical Modificationsof rRNAs
- Non-Radioactive In Vivo Labelling of RNA with 4-thio-uracil
- Translation Phases in Eukaryotes
- Differential Translation Activity using Bio-Orthogonal Non-Canonical Amino Acids Tagging (BONCAT) in Archaea
- Thermofluor-Based Analysis of Protein Integrity and Ligand Interactions
- In Vitro Assembly of a Fully Reconstituted Yeast Translation System for Studies of Initiation and Elongation Phases of Protein Synthesis.