Ribosome Biogenesis Methods and Protocols /

This Open Access volume provides comprehensive reviews and describes the latest techniques to study eukaryotic ribosome biogenesis. For more than 50 years ribosomes are a major research topic. Our knowledge about ribosome biogenesis and function such as transcription, mRNA modification, and translat...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
निगमित लेखक: SpringerLink (Online service)
अन्य लेखक: Entian, Karl-Dieter (संपादक)
स्वरूप: इलेक्ट्रोनिक ई-पुस्तक
भाषा:अंग्रेज़ी
प्रकाशित: New York, NY : Springer US : Imprint: Humana, 2022.
संस्करण:1st ed. 2022.
श्रृंखला:Methods in Molecular Biology, 2533
विषय:
ऑनलाइन पहुंच:Link to Metadata
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विषय - सूची:
  • A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity across the Tree of Life
  • Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes
  • Analysis of Yeast RNAP I Transcription of Nucleosomal Templates In Vitro
  • Specialization of RNA Polymerase I in Comparison to Other Nuclear RNA Polymerases of Saccharomyces cerevisiae
  • Structural Studies of Eukaryotic RNA Polymerase I using Cryo-Electron Microscopy
  • Preparation of RNA Polymerase Complexes for their Analysis by Single Particle Cryo-Electron Microscopy
  • Eukaryotic Ribosome Assembly and Nucleo-Cytoplasmic Transport
  • Tethered MNase Structure Probing as Versatile Technique for Analyzing RNPs using Tagging Cassettes for Homologous Recombination in Saccharomyces cerevisiae
  • Chemical Modifications of Ribosomal RNA
  • In Vitro Selection of Deoxyribozymes for the Detection of RNA Modifications
  • Mapping of the Chemical Modificationsof rRNAs
  • Non-Radioactive In Vivo Labelling of RNA with 4-thio-uracil
  • Translation Phases in Eukaryotes
  • Differential Translation Activity using Bio-Orthogonal Non-Canonical Amino Acids Tagging (BONCAT) in Archaea
  • Thermofluor-Based Analysis of Protein Integrity and Ligand Interactions
  • In Vitro Assembly of a Fully Reconstituted Yeast Translation System for Studies of Initiation and Elongation Phases of Protein Synthesis.