Low-Cost Methods for Molecular Characterization of Mutant Plants Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols /

• Main Authors: Till, Bradley J. (Author), Jankowicz-Cieslak, Joanna (Author), Huynh, Owen A. (Author), Beshir, Mayada M. (Author), Laport, Robert G. (Author), Hofinger, Bernhard J. (Author)
• Corporate Author: SpringerLink (Online service)
• Format: Electronic eBook
• Language: English
• Published: Cham : Springer International Publishing : Imprint: Springer, 2015.
• Edition: 1st ed. 2015.
• Subjects: Plant biotechnology.; Biology > Technique.; Biomaterials.; Nucleic acids.; Plant Biotechnology.; Biological Techniques.; Nucleic Acid.
• Online Access: Link to Metadata

Summary

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

Physical Description

X, 35 p. 9 illus., 3 illus. in color. online resource.

ISBN

9783319162591

DOI

10.1007/978-3-319-16259-1

Access

Open Access