Cell-Free Synthetic Biology

• Main Author: Hong, Seok Hoon (auth)
• Format: Electronic Book Chapter
• Language: English
• Published: MDPI - Multidisciplinary Digital Publishing Institute 2020
• Subjects: History of engineering & technology; genomically engineered E. coli; n/a; in vitro transcription-translation (TX-TL); tPa; tissue plasminogen activator; in vitro translation; enzymes; DNA origami; antimicrobials; cell-free metabolic engineering (CFME); cell-free transcription-translation; in vitro protein synthesis; cell-free metabolic engineering; E. coli crude extract preparation; chaperones; ischemic stroke; microsomes; sonication; cell-free protein synthesis; growth factors; artificial cell; unnatural protein; cell-free synthetic biology; rapid prototyping; drug development; unnatural amino acid; eukaryotic lysates; cell-free protein expression (CFPE); riboregulator; synthetic biology; mathematical model; CFPS; cell-free protein synthesis (CFPS); bioconversion platform; colicins; protein production
• Online Access: DOAB: download the publication; DOAB: description of the publication

Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering too...