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Cell-Free Synthetic Biology

Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering too...

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Bibliographic Details
Main Author: Hong, Seok Hoon (auth)
Format: Electronic Book Chapter
Language:English
Published: MDPI - Multidisciplinary Digital Publishing Institute 2020
Subjects:
History of engineering & technology
genomically engineered E. coli
n/a
in vitro transcription-translation (TX-TL)
tPa
tissue plasminogen activator
in vitro translation
enzymes
DNA origami
antimicrobials
cell-free metabolic engineering (CFME)
cell-free transcription-translation
in vitro protein synthesis
cell-free metabolic engineering
E. coli crude extract preparation
chaperones
ischemic stroke
microsomes
sonication
cell-free protein synthesis
growth factors
artificial cell
unnatural protein
cell-free synthetic biology
rapid prototyping
drug development
unnatural amino acid
eukaryotic lysates
cell-free protein expression (CFPE)
riboregulator
synthetic biology
mathematical model
CFPS
cell-free protein synthesis (CFPS)
bioconversion platform
colicins
protein production
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Description
Summary:Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas.
Physical Description:1 electronic resource (152 p.)
ISBN:books978-3-03928-023-0
9783039280230
9783039280223
Access:Open Access

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