Parallel imaging of Drosophila embryos for quantitative analysis of genetic perturbations of the Ras pathway

• Main Authors: Yogesh Goyal (Author), Thomas J. Levario (Author), Henry H. Mattingly (Author), Susan Holmes (Author), Stanislav Y. Shvartsman (Author), Hang Lu (Author)
• Format: Book
• Published: The Company of Biologists, 2017-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The Ras pathway patterns the poles of the Drosophila embryo by downregulating the levels and activity of a DNA-binding transcriptional repressor Capicua (Cic). We demonstrate that the spatiotemporal pattern of Cic during this signaling event can be harnessed for functional studies of mutations in th...