Evaluation of conventional and four real-time PCR methods for the detection of Leishmania on field-collected samples in Ethiopia.

• Main Authors: Behailu Merdekios (Author), Myrthe Pareyn (Author), Dagimawie Tadesse (Author), Nigatu Eligo (Author), Mekibib Kassa (Author), Bart K M Jacobs (Author), Herwig Leirs (Author), Jean-Pierre Van Geertruyden (Author), Johan van Griensven (Author), Guy Caljon (Author), Lieselotte Cnops (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2021-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In most low-resource settings, microscopy still is the standard method for diagnosis of cutaneous leishmaniasis, despite its limited sensitivity. In Ethiopia, the more sensitive molecular methods are not yet routinely used. This study compared five PCR methods with microscopy on two sample types col...