A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cells

• Main Authors: Seung Bum Park (Author), Takuro Uchida (Author), Samantha Tilson (Author), Zongyi Hu (Author), Christopher D. Ma (Author), Madeleine Leek (Author), Michael Eichner (Author), So Gun Hong (Author), T. Jake Liang (Author)
• Format: Book
• Published: Elsevier, 2022-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The CRISPR-Cas9 system has emerged as a powerful and efficient tool for genome editing. An important drawback of the CRISPR-Cas9 system is the constitutive endonuclease activity when Cas9 endonuclease and its sgRNA are co-expressed. This constitutive activity results in undesirable off-target effect...