Affinity Purification of Tumor Necrosis Factor-α Expressed in Raji Cells by Produced scFv Antibody Coupled CNBr-Activated Sepharose

• Main Authors: Safar Farajnia (Author), AliAkbar Movassaghpour (Author), Behzad Baradaran (Author), Mohammad Nouri (Author), Jafar Majidi Zolbanin (Author), Jalal Abdolalizadeh (Author), Yadollah Omidi (Author)
• Format: Book
• Published: Tabriz University of Medical Sciences, 2013-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Purpose: Recombinant tumor necrosis factor-alpha (TNF-α) has been utilized as an antineoplastic agent for the treatment of patients with melanoma and sarcoma. It targets tumor cell antigens by impressing tumor-associated vessels. Protein purification with affinity chromatography has been widely used...