Development of a Combined Lipid-Based Nanoparticle Formulation for Enhanced siRNA Delivery to Vascular Endothelial Cells

• Egile Nagusiak: Yutong He (Egilea), Dongdong Bi (Egilea), Josée A. Plantinga (Egilea), Grietje Molema (Egilea), Jeroen Bussmann (Egilea), Jan A. A. M. Kamps (Egilea)
• Formatua: Liburua
• Argitaratua: MDPI AG, 2022-09-01T00:00:00Z.
• Gaiak: article
• Sarrera elektronikoa: Connect to this object online.

Low transfection efficiency in endothelial cells (EC) is still a bottleneck for the majority of siRNA-based vascular delivery approaches. In this work, we developed a lipid-based nanoparticle (LNP) formulation based on a combination of a permanently charged cationic lipid-DOTAP and a conditionally i...