High level expression of recombinant human growth hormone in Escherichia coli: crucial role of translation initiation region

• Main Authors: Mahsa Ghavim (Author), Khalil Abnous (Author), Fatemeh Arasteh (Author), Sahar Taghavi (Author), Maryam Sadat Nabavinia (Author), Mona Alibolandi (Author), Mohammad Ramezani (Author)
• Format: Book
• Published: Wolters Kluwer Medknow Publications, 2017-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

For high-throughput production of recombinant protein in Escherichia coli (E. coli ), besides important parameters such as efficient vector with strong promoter and compatible host, other important issues including codon usage, rare codons, and GC content specially at N-terminal region should be con...