Tumor Necrosis Factor-α Mediates the Blood-Brain Barrier Dysfunction Induced by Activated Microglia in Mouse Brain Microvascular Endothelial Cells

• Main Authors: Tsuyoshi Nishioku (Author), Junichi Matsumoto (Author), Shinya Dohgu (Author), Noriko Sumi (Author), Kenji Miyao (Author), Fuyuko Takata (Author), Hideki Shuto (Author), Atsushi Yamauchi (Author), Yasufumi Kataoka (Author)
• Format: Book
• Published: Elsevier, 2010-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

The present study was designed to elucidate the involvement of tumor necrosis factor-α (TNF-α) release from activated microglia in the induction of blood-brain barrier (BBB) dysfunction in an in vitro co-culture system with mouse brain capillary endothelial cells (MBEC4) and microglia. Lipopolysaccharide (LPS)-activated microglia increased the permeability of MBEC4 cells to sodium-fluorescein, and this hyper-permeability was blocked by a neutralizing antibody against TNF-α. LPS stimulated microglia to facilitate TNF-α release. These findings suggested that TNF-α released from activated microglia is attributable to BBB dysfunction. Keywords:: microglia, tumor necrosis factor-α (TNF-α), blood-brain barrier

Item Description

1347-8613
10.1254/jphs.09292SC