Modification of the medium volume and gel substrate under in vitro culture conditions improves growth of porcine oocytes derived from early antral follicles

• Main Authors: Hidenori SHIBAHARA (Author), Yasuhisa MUNAKATA (Author), Ai ISHIGURO (Author), Koumei SHIRASUNA (Author), Takehito KUWAYAMA (Author), Hisataka IWATA (Author)
• Format: Book
• Published: The Society for Reproduction and Development, 2019-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

This study compared the effects of different volumes of culture medium for the in vitro growth of oocytes derived from porcine early antral follicles (EAFs). Oocyte granulosa cell complexes (OGCs) were collected from EAFs (0.5-0.7 mm in diameter) and individually cultured for 14 days. When OGCs were...