Improving of pelB-Secreted MPT64 protein released by Escherichia coli BL21 (DE3) using Triton X-100 and Tween-80

• Main Authors: Sri Agung Fitri Kusuma (Author), Toto Subroto (Author), Ida Parwati (Author), Yaya Rukayadi (Author), Muhammad Fadhlillah (Author), Ruth Michellee Pardede (Author), Alif Virisy Berlian (Author), Gina Sabila (Author)
• Format: Book
• Published: Wolters Kluwer Medknow Publications, 2022-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

pelB has been known as a successful signal peptide to translocate the protein target extracellularly in the Escherichia coli system. However, in our previous study, the yield of MPT64 protein extracellular recovery was still low and plenty of this protein was remain trapped in cytoplasm and periplas...