Expression and Biological Evaluation of an Engineered Recombinant L-asparaginase Designed by In Silico Method Based on Sequence of the Enzyme from Escherichia coli

• Main Authors: Mahrokh Dastmalchi (Author), Mahdiyeh Alizadeh (Author), Omid Jamshidi-Kandjan (Author), Hassan Rezazadeh (Author), Maryam Hamzeh-Mivehroud (Author), Mohammad M Farajollahi (Author), Siavoush Dastmalchi (Author)
• Format: Book
• Published: Tabriz University of Medical Sciences, 2023-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Purpose: Medical usage of L-asparaginase (ASNase), the first-line of acute lymphoblastic leukemia treatment, is linked to allergic responses and toxicities, which necessitates the development of new bio-better ASNases. The aim of the current study was in silico design of a novel ASNase with predicte...