Expression and Biological Evaluation of an Engineered Recombinant L-asparaginase Designed by In Silico Method Based on Sequence of the Enzyme from Escherichia coli

• Autors principals: Mahrokh Dastmalchi (Autor), Mahdiyeh Alizadeh (Autor), Omid Jamshidi-Kandjan (Autor), Hassan Rezazadeh (Autor), Maryam Hamzeh-Mivehroud (Autor), Mohammad M Farajollahi (Autor), Siavoush Dastmalchi (Autor)
• Format: Llibre
• Publicat: Tabriz University of Medical Sciences, 2023-11-01T00:00:00Z.
• Matèries: article
• Accés en línia: Connect to this object online.

Purpose: Medical usage of L-asparaginase (ASNase), the first-line of acute lymphoblastic leukemia treatment, is linked to allergic responses and toxicities, which necessitates the development of new bio-better ASNases. The aim of the current study was in silico design of a novel ASNase with predicte...