New primers for methylation-specific polymerase chain reaction enhance specificity of detecting STAT1 methylation

• Main Authors: Ming-Cheng Chang (Author), Ying-Cheng Chiang (Author), Chih-Ming Ho (Author), Yu-Li Chen (Author), Chi-An Chen (Author), Wen-Fang Cheng (Author), Cheng-Yang Chou (Author)
• Format: Book
• Published: Elsevier, 2012-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Objective: Signal transducer and activator of transcription (STAT)1 is a key tumor suppressor, which is always methylated in a variety of human cancers. However, nonspecific primers for the detection of specific promoter hypermethylation of STAT1 gene can lead to false-positive or false-negative res...