New primers for methylation-specific polymerase chain reaction enhance specificity of detecting STAT1 methylation

• Autors principals: Ming-Cheng Chang (Autor), Ying-Cheng Chiang (Autor), Chih-Ming Ho (Autor), Yu-Li Chen (Autor), Chi-An Chen (Autor), Wen-Fang Cheng (Autor), Cheng-Yang Chou (Autor)
• Format: Llibre
• Publicat: Elsevier, 2012-03-01T00:00:00Z.
• Matèries: article
• Accés en línia: Connect to this object online.

Objective: Signal transducer and activator of transcription (STAT)1 is a key tumor suppressor, which is always methylated in a variety of human cancers. However, nonspecific primers for the detection of specific promoter hypermethylation of STAT1 gene can lead to false-positive or false-negative res...