pEVL: A Linear Plasmid for Generating mRNA IVT Templates With Extended Encoded Poly(A) Sequences

• Main Authors: Alexandra E Grier (Author), Stephen Burleigh (Author), Jaya Sahni (Author), Courtnee A Clough (Author), Victoire Cardot (Author), Dongwook C Choe (Author), Michelle C Krutein (Author), David J Rawlings (Author), Michael C Jensen (Author), Andrew M Scharenberg (Author), Kyle Jacoby (Author)
• Format: Book
• Published: Elsevier, 2016-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Increasing demand for large-scale synthesis of in vitro transcribed (IVT) mRNA is being driven by the increasing use of mRNA for transient gene expression in cell engineering and therapeutic applications. An important determinant of IVT mRNA potency is the 3' polyadenosine (poly(A)) tail, the l...