Optimized strategy for real-time qPCR detection of Onchocerca volvulus DNA in pooled Simulium sp. blackfly vectors.

• Main Authors: Mary Doherty (Author), Jessica R Grant (Author), Nils Pilotte (Author), Sasisekhar Bennuru (Author), Kerstin Fischer (Author), Peter U Fischer (Author), Sara Lustigman (Author), Thomas B Nutman (Author), Kenneth Pfarr (Author), Achim Hoerauf (Author), Thomas R Unnasch (Author), Hassan K Hassan (Author), Samuel Wanji (Author), Patrick J Lammie (Author), Eric Ottesen (Author), Charles Mackenzie (Author), Steven A Williams (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2023-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<h4>Background</h4>Onchocerca volvulus is a filarial parasite that is a major cause of dermatitis and blindness in endemic regions primarily in sub-Saharan Africa. Widespread efforts to control the disease caused by O. volvulus infection (onchocerciasis) began in 1974 and in recent years...