Optimization of Propidium Monoazide qPCR (Viability-qPCR) to Quantify the Killing by the <i>Gardnerella</i>-Specific Endolysin PM-477, Directly in Vaginal Samples from Women with Bacterial Vaginosis

• Main Authors: Agnieszka Latka (Author), Leen Van Simaey (Author), Marijke Reynders (Author), Piet Cools (Author), Tess Rogier (Author), Barbara Lebbe (Author), Lorenzo Corsini (Author), Christine Landlinger (Author), Mario Vaneechoutte (Author)
• Format: Book
• Published: MDPI AG, 2022-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Quantification of the number of living cells in biofilm or after eradication treatments of biofilm, is problematic for different reasons. We assessed the performance of pre-treatment of DNA, planktonic cells and ex vivo vaginal biofilms of <i>Gardnerella</i> with propidium monoazide (PMA...