Separation and simultaneous quantitation of PGF2α and its epimer 8-iso-PGF2α using modifier-assisted differential mobility spectrometry tandem mass spectrometry
Because many therapeutic agents are contaminated by epimeric impurities or form epimers as a result of metabolism, analytical tools capable of determining epimers are increasingly in demand. This article is a proof-of-principle report of a novel DMS-MS/MS method to separate and simultaneously quanti...
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| Main Authors: | , , , , , , , |
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| Format: | Book |
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Elsevier,
2018-03-01T00:00:00Z.
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| Online Access: | Connect to this object online. |
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| Summary: | Because many therapeutic agents are contaminated by epimeric impurities or form epimers as a result of metabolism, analytical tools capable of determining epimers are increasingly in demand. This article is a proof-of-principle report of a novel DMS-MS/MS method to separate and simultaneously quantify epimers, taking PGF2α and its 8-epimer, 8-iso-PGF2α, as an example. Good accuracy and precision were achieved in the range of 10-500 ng/mL with a run time of only 1.5 min. Isopropanol as organic modifier facilitated a good combination of sensitivity and separation. The method is the first example of the quantitation of epimers without chromatographic separation. KEY WORDS: Differential mobility spectrometry, Mass spectrometry, Epimer, PGF2α, 8-iso-PGF2α |
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| Item Description: | 2211-3835 10.1016/j.apsb.2018.01.011 |