Epitope mapping of Ebola virus dominant and subdominant glycoprotein epitopes facilitates construction of an epitope-based DNA vaccine able to focus the antibody response in mice

• Main Authors: Daniel A. J. Mitchell (Author), Lesley C. Dupuy (Author), Mariano Sanchez-Lockhart (Author), Gustavo Palacios (Author), Jaap W. Back (Author), Katya Shimanovskaya (Author), Sidhartha Chaudhury (Author), Daniel R. Ripoll (Author), Anders Wallqvist (Author), Connie S. Schmaljohn (Author)
• Format: Book
• Published: Taylor & Francis Group, 2017-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We performed epitope mapping studies on the major surface glycoprotein (GP) of Ebola virus (EBOV) using Chemically Linked Peptides on Scaffolds (CLIPS), which form linear and potential conformational epitopes. This method identified monoclonal antibody epitopes and predicted additional epitopes reco...