Analysis and Optimization of Conditions for the Use of 2',7'-Dichlorofluorescein Diacetate in Cultured Hepatocytes

• Main Authors: Megan J. Reiniers (Author), Lianne R. de Haan (Author), Laurens F. Reeskamp (Author), Mans Broekgaarden (Author), Rowan F. van Golen (Author), Michal Heger (Author)
• Format: Book
• Published: MDPI AG, 2021-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Numerous liver pathologies encompass oxidative stress as molecular basis of disease. The use of 2',7'-dichlorodihydrofluorescein-diacetate (DCFH₂-DA) as fluorogenic redox probe is problematic in liver cell lines because of membrane transport proteins that interfere w...