Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies.

• Main Authors: Sascha Knauf (Author), Simone Lüert (Author), David Šmajs (Author), Michal Strouhal (Author), Idrissa S Chuma (Author), Sieghard Frischmann (Author), Mohammed Bakheit (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2018-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We show proof of concept for gene targets (polA, tprL, and TP_0619) that can be used in loop-mediated isothermal amplification (LAMP) assays to rapidly differentiate infection with any of the three Treponema pallidum subspecies (pallidum (TPA), pertenue (TPE), and endemicum (TEN)) and which are know...