Monitoring of the Parasite Load in the Digestive Tract of Rhodnius prolixus by Combined qPCR Analysis and Imaging Techniques Provides New Insights into the Trypanosome Life Cycle.

• Main Authors: Felipe de Almeida Dias (Author), Barbara Guerra (Author), Larissa Rezende Vieira (Author), Hugo Diego Perdomo (Author), Ana Caroline Paiva Gandara (Author), Raquel Juliana Vionette do Amaral (Author), Renata Estebanez Vollú (Author), Suzete Araujo Oliveira Gomes (Author), Flavio Alves Lara (Author), Marcos Henrique Ferreira Sorgine (Author), Emiliano Medei (Author), Pedro Lagerblad de Oliveira (Author), Didier Salmon (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2015-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Here we report the monitoring of the digestive tract colonization of Rhodnius prolixus by Trypanosoma cruzi using an accurate determination of the parasite load by qPCR coupled with fluorescence and bioluminescence imaging (BLI). These complementary methods revealed critical steps necessary for the...