Formulation, inflammation, and RNA sensing impact the immunogenicity of self-amplifying RNA vaccines

• Main Authors: John S. Tregoning (Author), David C. Stirling (Author), Ziyin Wang (Author), Katie E. Flight (Author), Jonathan C. Brown (Author), Anna K. Blakney (Author), Paul F. McKay (Author), Robert F. Cunliffe (Author), Valarmathy Murugaiah (Author), Christopher B. Fox (Author), Mitchell Beattie (Author), Ying K. Tam (Author), Cecilia Johansson (Author), Robin J. Shattock (Author)
• Format: Book
• Published: Elsevier, 2023-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

To be effective, RNA vaccines require both in situ translation and the induction of an immune response to recruit cells to the site of immunization. These factors can pull in opposite directions with the inflammation reducing expression of the vaccine antigen. We investigated how formulation affects...