Site-Specific Structural Changes in Long-Term-Stressed Monoclonal Antibody Revealed with DEPC Covalent-Labeling and Quantitative Mass Spectrometry

• Main Authors: Manasi Gaikwad (Author), Florian Richter (Author), Rabea Götz (Author), Aline Dörrbaum (Author), Lena Schumacher (Author), Jason Tonillo (Author), Christian Frech (Author), Roland Kellner (Author), Carsten Hopf (Author)
• Format: Book
• Published: MDPI AG, 2023-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Studies of structural changes in mAbs under forced stress and storage conditions are essential for the recognition of degradation hotspots, which can be further remodeled to improve the stability of the respective protein. Herein, we used diethyl pyrocarbonate (DEPC)-based covalent labeling mass spe...