<i>S. aureus</i> Colonization, Biofilm Production, and Phage Susceptibility in Peritoneal Dialysis Patients

• Những tác giả chính: Karlis Racenis (Tác giả), Juta Kroica (Tác giả), Dace Rezevska (Tác giả), Lauris Avotins (Tác giả), Edgars Skuditis (Tác giả), Anna Popova (Tác giả), Ilze Puide (Tác giả), Viktorija Kuzema (Tác giả), Aivars Petersons (Tác giả)
• Định dạng: Sách
• Được phát hành: MDPI AG, 2020-09-01T00:00:00Z.
• Những chủ đề: article
• Truy cập trực tuyến: Connect to this object online.
• Notes: 10.3390/antibiotics9090582; 2079-6382
• Keywords: <i>S. aureus</i>; peritoneal dialysis; phage adaptation; biofilm; phage therapy; phenotypic trait; Therapeutics. Pharmacology; RM1-950
• Source: Antibiotics, Vol 9, Iss 9, p 582 (2020)

Peritonitis caused by <i>Staphylococcus</i><i>aureus</i> is of major importance in peritoneal dialysis (PD) patients due to its great virulence profile and biofilm formation ability. Bacteriophages are a potential tool to treat peritonitis resulting from biofilm-associated infections. We screened <i>S. aureus</i> colonization in 71 PD patients from the nasal cavity, groin, and PD exit-site regions and analyzed clinical outcomes in these patients. We performed biofilm-formation testing of different strains and compared the isolates of one patient to detect phenotypic differences in <i>S. aureus</i>. Phage cocktails were used to detect <i>S. aureus</i> in vitro susceptibility. An adaptation procedure was performed in cases of bacterial resistance. Around 30% of PD patients (<i>n</i> = 21) were found to be <i>S. aureus</i> carriers; from these, a total of 34 <i>S. aureus</i> strains were isolated, of which 61.8% (<i>n</i> = 21) produced a strong biofilm. Phenotypic differences in strain biofilm production were detected in eight patients out of ten. All strains were sensitive to commonly used antibiotics. Broadly positive phage lytic activity (100%) was observed in six cocktails out of seven, and bacterial resistance towards phages was overcome using adaptation. Overall phages showed a promising in vitro effect in biofilm-forming <i>S. aureus</i> strains.