<i>S. aureus</i> Colonization, Biofilm Production, and Phage Susceptibility in Peritoneal Dialysis Patients

• Autori principali: Karlis Racenis (Autore), Juta Kroica (Autore), Dace Rezevska (Autore), Lauris Avotins (Autore), Edgars Skuditis (Autore), Anna Popova (Autore), Ilze Puide (Autore), Viktorija Kuzema (Autore), Aivars Petersons (Autore)
• Natura: Libro
• Pubblicazione: MDPI AG, 2020-09-01T00:00:00Z.
• Soggetti: article
• Accesso online: Connect to this object online.
• Notes: 10.3390/antibiotics9090582; 2079-6382
• Keywords: <i>S. aureus</i>; peritoneal dialysis; phage adaptation; biofilm; phage therapy; phenotypic trait; Therapeutics. Pharmacology; RM1-950
• Source: Antibiotics, Vol 9, Iss 9, p 582 (2020)

Peritonitis caused by <i>Staphylococcus</i><i>aureus</i> is of major importance in peritoneal dialysis (PD) patients due to its great virulence profile and biofilm formation ability. Bacteriophages are a potential tool to treat peritonitis resulting from biofilm-associated infections. We screened <i>S. aureus</i> colonization in 71 PD patients from the nasal cavity, groin, and PD exit-site regions and analyzed clinical outcomes in these patients. We performed biofilm-formation testing of different strains and compared the isolates of one patient to detect phenotypic differences in <i>S. aureus</i>. Phage cocktails were used to detect <i>S. aureus</i> in vitro susceptibility. An adaptation procedure was performed in cases of bacterial resistance. Around 30% of PD patients (<i>n</i> = 21) were found to be <i>S. aureus</i> carriers; from these, a total of 34 <i>S. aureus</i> strains were isolated, of which 61.8% (<i>n</i> = 21) produced a strong biofilm. Phenotypic differences in strain biofilm production were detected in eight patients out of ten. All strains were sensitive to commonly used antibiotics. Broadly positive phage lytic activity (100%) was observed in six cocktails out of seven, and bacterial resistance towards phages was overcome using adaptation. Overall phages showed a promising in vitro effect in biofilm-forming <i>S. aureus</i> strains.