Development and analytical validation of real-time PCR for the detection of Streptococcus agalactiae in pregnant women

• Päätekijät: Daniel F. Escobar (Tekijä), Diego A. Diaz-Dinamarca (Tekijä), Carlos F. Hernández (Tekijä), Daniel A. Soto (Tekijä), Ricardo A. Manzo (Tekijä), Pedro I. Alarcón (Tekijä), Camila H. Pinto (Tekijä), Diego N. Bastias (Tekijä), Carolayn N. Oberg-Bravo (Tekijä), Robert Rojas (Tekijä), Sebastián E. Illanes (Tekijä), Alexis M. Kalergis (Tekijä), Abel E. Vasquez (Tekijä)
• Aineistotyyppi: Kirja
• Julkaistu: BMC, 2020-06-01T00:00:00Z.
• Aiheet: article
• Linkit: Connect to this object online.

Abstract Background Group B Streptococcus (GBS) is the leading cause of invasive neonatal infection. In this study, we aimed to evaluate the analytical validation of qualitative real-time polymerase chain reaction (qPCR) as a means to detect GBS. Methods Genomic DNA (gDNA) was purified from 12 ATCC...