Development and analytical validation of real-time PCR for the detection of Streptococcus agalactiae in pregnant women

• Main Authors: Daniel F. Escobar (Author), Diego A. Diaz-Dinamarca (Author), Carlos F. Hernández (Author), Daniel A. Soto (Author), Ricardo A. Manzo (Author), Pedro I. Alarcón (Author), Camila H. Pinto (Author), Diego N. Bastias (Author), Carolayn N. Oberg-Bravo (Author), Robert Rojas (Author), Sebastián E. Illanes (Author), Alexis M. Kalergis (Author), Abel E. Vasquez (Author)
• Format: Book
• Published: BMC, 2020-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Background Group B Streptococcus (GBS) is the leading cause of invasive neonatal infection. In this study, we aimed to evaluate the analytical validation of qualitative real-time polymerase chain reaction (qPCR) as a means to detect GBS. Methods Genomic DNA (gDNA) was purified from 12 ATCC...