Development and analytical validation of real-time PCR for the detection of Streptococcus agalactiae in pregnant women

• Autores principales: Daniel F. Escobar (Autor), Diego A. Diaz-Dinamarca (Autor), Carlos F. Hernández (Autor), Daniel A. Soto (Autor), Ricardo A. Manzo (Autor), Pedro I. Alarcón (Autor), Camila H. Pinto (Autor), Diego N. Bastias (Autor), Carolayn N. Oberg-Bravo (Autor), Robert Rojas (Autor), Sebastián E. Illanes (Autor), Alexis M. Kalergis (Autor), Abel E. Vasquez (Autor)
• Formato: Libro
• Publicado: BMC, 2020-06-01T00:00:00Z.
• Materias: article
• Acceso en línea: Connect to this object online.

Abstract Background Group B Streptococcus (GBS) is the leading cause of invasive neonatal infection. In this study, we aimed to evaluate the analytical validation of qualitative real-time polymerase chain reaction (qPCR) as a means to detect GBS. Methods Genomic DNA (gDNA) was purified from 12 ATCC...