The proximity of the N- and C- termini of bovine knob domains enable engineering of target specificity into polypeptide chains

• Main Authors: Alice Hawkins (Author), Callum Joyce (Author), Kevin Brady (Author), Adam Hold (Author), Alan Smith (Author), Michael Knight (Author), Conor Howard (Author), Jean van den Elsen (Author), Alastair D.G. Lawson (Author), Alex Macpherson (Author)
• Format: Book
• Published: Taylor & Francis Group, 2022-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Cysteine-rich knob domains can be isolated from the ultralong heavy-chain complementarity-determining region (CDR) 3, which are unique to a subset of bovine antibodies, to create antibody fragments of ~4 kDa. Advantageously, the N- and C- termini of these small binding domains are in close proximity...