Real-time monitoring of rhamnose induction effect on the expression of mpt64 gene fused with pelB signal peptide in Escherichia coli BL21 (DE3)

• Main Authors: Sri Agung Fitri Kusuma (Author), Ida Parwati (Author), Toto Subroto (Author), Yaya Rukayadi (Author), Tina Rostinawati (Author), Muhammad Yusuf (Author), Muhammad Fadhlillah (Author), Laily D Tanti (Author), Risa R Ahyudanari (Author)
• Format: Book
• Published: Wolters Kluwer Medknow Publications, 2020-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In this research, Escherichia coli BL21 (DE3) harboring an expression vector constructed with a rhamnose-inducible promoter and a pelB signal peptide was used as a host cell to produce MPT64 protein. The objective of this research was to figure out the optimum time of mpt64 gene expression through r...