CRISPR/Cas9-AAV Mediated Knock-in at NRL Locus in Human Embryonic Stem Cells

• Main Authors: Xianglian Ge (Author), Haitao Xi (Author), Fayu Yang (Author), Xiao Zhi (Author), Yanghua Fu (Author), Ding Chen (Author), Ren-He Xu (Author), Ge Lin (Author), Jia Qu (Author), Junzhao Zhao (Author), Feng Gu (Author)
• Format: Book
• Published: Elsevier, 2016-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Clustered interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome engineering technologies are sparking a new revolution in biological research. This technology efficiently induces DNA double strand breaks at the targeted genomic sequence and results in inde...