No detection of c-kit gene mutations in exons 9, 11, 13 and 17 and low CD117 expression in plaque-stage mycosis fungoides

• Main Authors: David Aldo De Luca (Author), Paula Andrea Enz (Author), Ricardo Luis Galimberti (Author), Adriana Raquel Rinflerch (Author)
• Format: Book
• Published: Sociedade Brasileira de Dermatologia, 2018-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.
• Notes: 0365-0596; 10.1590/abd1806-4841.20187930
• Keywords: Mycosis fungoides; Proto-oncogene proteins c-kit; Stem cell factor; Dermatology; RL1-803
• Source: Anais Brasileiros de Dermatologia, Vol 93, Iss 6, Pp 913-915 (2018)

Abstract: The growth factor receptor c-kit (CD117) is expressed in immature T-cells and in some advanced forms of mycosis fungoides. c-kit gene mutation results in unrestricted neoplastic proliferation. We aimed to detect by PCR the most frequent exon mutations in seventeen plaque-stage MF patients, in their perilesional skin and in healthy skin donors. We secondarily evaluated CD117 expression by immunohistochemistry in plaque-stage and tumor-stage MF. We detected no mutation in c-kit gene and low CD117 expression was confirmed on atypical cells in one patient. Complete c-kit exon and intron sequences should be assessed and more sensitive sequencing method could be also applied.