Development of PCR based approach to detect potential mosaicism in porcine embryos

• Main Authors: Jongki Cho (Author), Kyungjun Uh (Author), Junghyun Ryu (Author), Xun Fang (Author), Seonggyu Bang (Author), Kiho Lee (Author)
• Format: Book
• Published: The Korean Society of Animal Reproduction and Biotechnology, 2020-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Direct injection of genome editing tools such as CRISPR/Cas9 system into developing embryos has been widely used to generate genetically engineered pigs. The approach allows us to produce pigs carrying targeted modifications at high efficiency without having to apply somatic cell nuclear transfer. H...