Generation of Hutat2:Fc Knockin Primary Human Monocytes Using CRISPR/Cas9

• Main Authors: Bowen Wang (Author), Jiahui Zuo (Author), Wenzhen Kang (Author), Qianqi Wei (Author), Jianhui Li (Author), Chunfu Wang (Author), Zhihui Liu (Author), Yuanan Lu (Author), Yan Zhuang (Author), Bianli Dang (Author), Qing Liu (Author), Wen Kang (Author), Yongtao Sun (Author)
• Format: Book
• Published: Elsevier, 2018-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The ability of monocytes to travel through the bloodstream, traverse tissue barriers, and aggregate at disease sites endows these cells with the attractive potential to carry therapeutic genes into the nervous system. However, gene editing in primary human monocytes has long been a challenge. Here,...