Establishment of multiplex RT-PCR to detect fusion genes for the diagnosis of Ewing sarcoma

• Main Authors: Hitomi Ueno-Yokohata (Author), Hajime Okita (Author), Keiko Nakasato (Author), Chikako Kiyotani (Author), Motohiro Kato (Author), Kimikazu Matsumoto (Author), Nobutaka Kiyokawa (Author), Atsuko Nakazawa (Author), Takako Yoshioka (Author)
• Format: Book
• Published: BMC, 2021-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Background Detection of the tumor-specific EWSR1/FUS-ETS fusion gene is essential to diagnose Ewing sarcoma. Reverse transcription-polymerase chain reaction (RT-PCR) and fluorescence in situ hybridization are commonly used to detect the fusion gene, and assays using next-generation sequenci...