Rat brain polyamines: an analytical method validation Poliaminas no cérebro do rato: validação de método analítico

• Main Authors: Valdomiro de Freitas Sampaio (Author), Gilberto J. Padovan (Author), Julio Sérgio Marchini (Author), Luiz Marcellino de Oliveira (Author), Sebastião Sousa Almeida (Author)
• Format: Book
• Published: Sociedade Brasileira de Patologia Clínica, 2010-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The validation of the analytical technique for the determination of polyamines in cerebral tissue using HPLC based on o-phthalaldehyde post-column derivatization is described. The polyamines were separated in a LiChrospher100 RP18 column. Elution gradient was formed with two mobile phases: A (sodium...