Methods to determine the transcriptomes of trypanosomes in mixtures with mammalian cells: the effects of parasite purification and selective cDNA amplification.

• Main Authors: Julius Mulindwa (Author), Abeer Fadda (Author), Clementine Merce (Author), Enoch Matovu (Author), John Enyaru (Author), Christine Clayton (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2014-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Patterns of gene expression in cultured Trypanosoma brucei bloodstream and procyclic forms have been extensively characterized, and some comparisons have been made with trypanosomes grown to high parasitaemias in laboratory rodents. We do not know, however, to what extent these transcriptomes resemb...