Genome-wide activation screens to increase adeno-associated virus production

• Egile Nagusiak: Christopher R. Barnes (Egilea), Hyuncheol Lee (Egilea), David S. Ojala (Egilea), Kazuomori K. Lewis (Egilea), Prajit Limsirichai (Egilea), David V. Schaffer (Egilea)
• Formatua: Liburua
• Argitaratua: Elsevier, 2021-12-01T00:00:00Z.
• Gaiak: article
• Sarrera elektronikoa: Connect to this object online.

We describe a genome-wide screening strategy to identify target genes whose modulation increases the capacity of a cell to produce recombinant adeno-associated viral (AAV) vector. Specifically, a single-guide RNA (sgRNA) library for a CRISPR-based genome-wide transcriptional activation screen was in...