Genome-wide activation screens to increase adeno-associated virus production

Genome-wide activation screens to increase adeno-associated virus production

We describe a genome-wide screening strategy to identify target genes whose modulation increases the capacity of a cell to produce recombinant adeno-associated viral (AAV) vector. Specifically, a single-guide RNA (sgRNA) library for a CRISPR-based genome-wide transcriptional activation screen was in...

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Bibliographic Details
Main Authors: Christopher R. Barnes (Author), Hyuncheol Lee (Author), David S. Ojala (Author), Kazuomori K. Lewis (Author), Prajit Limsirichai (Author), David V. Schaffer (Author)
Format: Book
Published: Elsevier, 2021-12-01T00:00:00Z.
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