On-column disulfide bond formation of monoclonal antibodies during Protein A chromatography eliminates low molecular weight species and rescues reduced antibodies

• Main Authors: Zhijun Tan (Author), Vivekh Ehamparanathan (Author), Tingwei Ren (Author), Peifeng Tang (Author), Laurel Hoffman (Author), June Kuang (Author), Peiran Liu (Author), Chao Huang (Author), Cheng Du (Author), Li Tao (Author), Letha Chemmalil (Author), Angela Lewandowski (Author), Sanchayita Ghose (Author), Zheng Jian Li (Author), Shijie Liu (Author)
• Format: Book
• Published: Taylor & Francis Group, 2020-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Disulfide bond reduction, which commonly occurs during monoclonal antibody (mAb) manufacturing processes, can result in a drug substance with high levels of low molecular weight (LMW) species that may fail release specifications because the drug's safety and the efficiency may be affected by th...