Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

• Main Authors: Xiao-Hui Zhang (Author), Louis Y Tee (Author), Xiao-Gang Wang (Author), Qun-Shan Huang (Author), Shi-Hua Yang (Author)
• Format: Book
• Published: Elsevier, 2015-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the functionality of genetic elements, creating genetically modified organisms as well as preclinical research of genetic disorders. However, the high frequency of off-target activity (≥50%)-RGEN (RNA-guided endonu...