Two Types of Cation Channel Activated by Stimulation of Muscarinic Receptors in Guinea-Pig Urinary Bladder Smooth Muscle
The present study, aiming to elucidate ion channel mechanisms underlying muscarinic receptor-induced depolarization, has characterized membrane currents induced by carbachol in single guinea-pig urinary bladder myocytes. Application of carbachol to cells that were voltage-clamped at − 50 mV produced...
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| Main Authors: | , , , , , , , |
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Elsevier,
2008-01-01T00:00:00Z.
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|---|---|---|---|
| 001 | doaj_1a0ff9e43bb0485ea7f11165bff84c1e | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Masayuki Yamamoto |e author |
| 700 | 1 | 0 | |a Toshihiro Unno |e author |
| 700 | 1 | 0 | |a Hayato Matsuyama |e author |
| 700 | 1 | 0 | |a Masanobu Kohda |e author |
| 700 | 1 | 0 | |a Noriyuki Masuda |e author |
| 700 | 1 | 0 | |a Masakazu Nishimura |e author |
| 700 | 1 | 0 | |a Toshiaki Ishii |e author |
| 700 | 1 | 0 | |a Seiichi Komori |e author |
| 245 | 0 | 0 | |a Two Types of Cation Channel Activated by Stimulation of Muscarinic Receptors in Guinea-Pig Urinary Bladder Smooth Muscle |
| 260 | |b Elsevier, |c 2008-01-01T00:00:00Z. | ||
| 500 | |a 1347-8613 | ||
| 500 | |a 10.1254/jphs.08138FP | ||
| 520 | |a The present study, aiming to elucidate ion channel mechanisms underlying muscarinic receptor-induced depolarization, has characterized membrane currents induced by carbachol in single guinea-pig urinary bladder myocytes. Application of carbachol to cells that were voltage-clamped at − 50 mV produced an atropine-sensitive, biphasic inward current consisting of an initial peak followed by a smaller sustained phase. Replacing the extracellular Na+ and intracellular Cl− with impermeable tris+ and glutamate−, respectively, demonstrated that the biphasic current is entirely composed of cation currents. Its initial peak phase was abolished by buffering intracellular Ca2+ to a constant level of 100 nM or depleting intracellular Ca2+ stores, and it was mimicked by the Ca2+ releaser caffeine. Ca2+ entry evoked by voltage steps in the sustained phase induced no noticeable change, indicating that this phase of cation current is insensitive to a rise of [Ca2+]i. These results demonstrate that muscarinic receptor stimulation invokes the openings of two types of cation channel, a Ca2+-activated and a receptor-operated type; the former channels are gated by a rise in [Ca2+]i upon intracellular Ca2+ release, and the latter are gated through other muscarinic receptor-coupled signal transduction mechanisms. Keywords:: cation channel, muscarinic receptor, carbachol, bladder smooth muscle, guinea pig | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Pharmacological Sciences, Vol 108, Iss 3, Pp 248-257 (2008) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S1347861319313313 | |
| 787 | 0 | |n https://doaj.org/toc/1347-8613 | |
| 856 | 4 | 1 | |u https://doaj.org/article/1a0ff9e43bb0485ea7f11165bff84c1e |z Connect to this object online. |