Combining random mutagenesis, structure-guided design and next-generation sequencing to mitigate polyreactivity of an anti-IL-21R antibody

• Main Authors: Sharon M. Campbell (Author), Joseph DeBartolo (Author), James R. Apgar (Author), Lydia Mosyak (Author), Virginie McManus (Author), Sonia Beyer (Author), Eric M. Bennett (Author), Matthew Lambert (Author), Orla Cunningham (Author)
• Format: Bog
• Udgivet: Taylor & Francis Group, 2021-01-01T00:00:00Z.
• Fag: article
• Online adgang: Connect to this object online.

Despite substantial technological advances in antibody library and display platform development, the number of approved biotherapeutics from displayed libraries remains limited. In vivo, 20-50% of peripheral B cells undergo a process of receptor editing, which modifies the variable and junctional re...