Validation of RNAi Silencing Efficiency Using Gene Array Data shows 18.5% Failure Rate across 429 Independent Experiments

• Principais autores: Gyöngyi Munkácsy (Autor), Zsófia Sztupinszki (Autor), Péter Herman (Autor), Bence Bán (Autor), Zsófia Pénzváltó (Autor), Nóra Szarvas (Autor), Balázs Győrffy (Autor)
• Formato: Livro
• Publicado em: Elsevier, 2016-01-01T00:00:00Z.
• Assuntos: article
• Acesso em linha: Connect to this object online.

No independent cross-validation of success rate for studies utilizing small interfering RNA (siRNA) for gene silencing has been completed before. To assess the influence of experimental parameters like cell line, transfection technique, validation method, and type of control, we have to validate the...