Validation of RNAi Silencing Efficiency Using Gene Array Data shows 18.5% Failure Rate across 429 Independent Experiments

• Hauptverfasser: Gyöngyi Munkácsy (Verfasst von), Zsófia Sztupinszki (Verfasst von), Péter Herman (Verfasst von), Bence Bán (Verfasst von), Zsófia Pénzváltó (Verfasst von), Nóra Szarvas (Verfasst von), Balázs Győrffy (Verfasst von)
• Format: Buch
• Veröffentlicht: Elsevier, 2016-01-01T00:00:00Z.
• Schlagworte: article
• Online-Zugang: Connect to this object online.

No independent cross-validation of success rate for studies utilizing small interfering RNA (siRNA) for gene silencing has been completed before. To assess the influence of experimental parameters like cell line, transfection technique, validation method, and type of control, we have to validate the...