Structure- and machine learning-guided engineering demonstrate that a non-canonical disulfide in an anti-PD-1 rabbit antibody does not impede antibody developability

• Main Authors: Wei-Ching Liang (Author), Hongkang Xi (Author), Dawei Sun (Author), Luigi D'Ascenzo (Author), Jonathan Zarzar (Author), Nicole Stephens (Author), Ryan Cook (Author), Yinyin Li (Author), Zhengmao Ye (Author), Marissa Matsumoto (Author), Jian Payandeh (Author), Matthieu Masureel (Author), Yan Wu (Author)
• Format: Book
• Published: Taylor & Francis Group, 2024-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

ABSTRACTRabbits produce robust antibody responses and have unique features in their antibody repertoire that make them an attractive alternative to rodents for in vivo discovery. However, the frequent occurrence of a non-canonical disulfide bond between complementarity-determining region (CDR) H1 (C...