15d-Prostaglandin J2 Enhancement of Nerve Growth Factor-Induced Neurite Outgrowth Is Blocked by the Chemoattractant Receptor- Homologous Molecule Expressed on T-Helper Type 2 Cells (CRTH2) Antagonist CAY10471 in PC12 Cells

• Main Authors: Michiyoshi Hatanaka (Author), Norihiro Shibata (Author), Norihito Shintani (Author), Ryota Haba (Author), Atsuko Hayata (Author), Hitoshi Hashimoto (Author), Akemichi Baba (Author)
• Format: Book
• Published: Elsevier, 2010-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

The chemoattractant receptor-homologous molecule expressed on T-helper type 2 cells (CRTH2) is the most recently identified prostaglandin (PG) receptor for both PGD2 and 15-deoxy-Δ12,14-PGJ2 (15d-PGJ2). We examined the mechanism by which 15d-PGJ2 enhances nerve growth factor (NGF)-induced neurite outgrowth in PC12 cells. CAY10471 (CRTH2 antagonist) inhibited both the neurite-promotion and p38 mitogen-activated protein (MAP) kinase phosphorylation induced by 15d-PGJ2. In contrast, 13,14-dihydro-15-keto-PGD2 (DK-PGD2) (selective CRTH2 agonist) stimulated its phosphorylation but failed to produce neurite-promoting effects. These suggest, for the first time, the action of 15d-PGJ2 is mediated by CRTH2, although the CRTH2 activation alone is insufficient for the underlying action. Keywords:: chemoattractant receptor-homologous molecule expressed on T-helper type 2 cells (CRTH2), nerve growth factor (NGF), neurite outgrowth

Item Description

1347-8613
10.1254/jphs.10001SC