Effect of trehalose on the preservation of freeze-dried mice spermatozoa at room temperature

• Main Authors: Daiyu ITO (Author), Sayaka WAKAYAMA (Author), Yuko KAMADA (Author), Ikue SHIBASAKI (Author), Satoshi KAMIMURA (Author), Masatoshi OOGA (Author), Teruhiko WAKAYAMA (Author)
• Format: Book
• Published: The Society for Reproduction and Development, 2019-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Freeze-drying of spermatozoa is a convenient and safe method to preserve mammalian genetic material without the use of liquid nitrogen or a deep freezer. However, freeze-dried spermatozoa (FD sperm) are not frequently used because of the low success rate of offspring after intracytoplasmic spermatoz...